Histone-modifying Enzymes Coregulated in Cancer – a phenomena that can be used for better drug design

4 of the 5 participants in the project, from left to right: Liza, Laura, William and Khademul

Recently epigenetic modifications have drawn lots of attention in the oncology field. Cancer is characterized by aberrant patterns of gene expression of multiple genes. These major shifts in gene expression are believed to be due to genetic and epigenetic changes. Read the rest of this entry »

Visualized ChIP & ChIPseq protocol

ChIP-seq (Chromatin Immunoprecipitation-and-Sequencing) has become a popular technique to find de novo and genome-wide binding sites of transcription factors. One of the basic yet laborious steps of this technique is the chromatin immunoprecipitation: If not even the positive control gives a signal after a 3-day experiment, and no mistake can be spotted in the preparation of the reagents or in following the steps of the protocol, most likely the protocol itself needs some optimization. Read the rest of this entry »

How to de-repress genes by ZRF1

Waddington's epigenetic landscape, from C.H. Waddington The strategy of genes: a discussion of some aspects of theoretical biology (Allen & Unwin, 1957)

Genomes contains the information necessary to form all the different types of cells present in the adult form. During embryonic development, the rapidly dividing cells in early embryo have a future of immense possibilities ahead. As exciting as this might sound, at some point they have to decide what kind of cells they will be in the future. Read the rest of this entry »